Department

Chemistry

Document Type

Article

Publication Date

1-28-2017

Abstract

While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Molecular modeling and experimental measurements were used to show that neither nucleic acid binding peptide selectively binds DNA sequences. Simulations and experiments also showed that changing lysines to arginines enhances DNA binding, suggesting that including additional guanidinium groups is a potential strategy to engineer more potent AMPs. Moreover, the lack of binding specificity may make it more difficult for bacteria to evolve resistance to these and other similar AMPs.

Comments

Post-print version of Sim, S. , Wang, P. , Beyer, B. N., Cutrona, K. J., Radhakrishnan, M. L. and Elmore, D. E. (2017), Investigating the nucleic acid interactions of histone‐derived antimicrobial peptides. FEBS Lett, 591: 706-717. doi:10.1002/1873-3468.12574

Final published version can be found here: https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.12574

Version

Post-print

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