Department

Biological Sciences, Chemistry

Document Type

Article

Publication Date

7-18-2016

Abstract

Studies attempting to characterize the membrane translocation of antimicrobial and cellpenetrating peptides frequently are limited by the resolution of conventional light microscopy. This study shows that spheroplasts provide a valuable approach to overcome these limits. Spheroplasts produce less ambiguous images and allow for more systematic analyses of localization. Data collected with spheroplasts are consistent with studies using normal bacterial cells and imply that a particular peptide may not always follow the same mechanism of action.

Comments

Post-print version of Wei L, LaBouyer MA, Darling LEO, Elmore DE. 2016. Bacterial spheroplasts as a model for visualizing membrane translocation of antimicrobial peptides. Antimicrob Agents Chemother 60:6350–6352. doi:10.1128/AAC.01008-16.

Final version can be found here:
http://aac.asm.org/content/60/10/6350.full

Version

Post-print

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