Biological Sciences, Chemistry
Studies attempting to characterize the membrane translocation of antimicrobial and cellpenetrating peptides frequently are limited by the resolution of conventional light microscopy. This study shows that spheroplasts provide a valuable approach to overcome these limits. Spheroplasts produce less ambiguous images and allow for more systematic analyses of localization. Data collected with spheroplasts are consistent with studies using normal bacterial cells and imply that a particular peptide may not always follow the same mechanism of action.
Wei L, LaBouyer MA, Darling LEO, Elmore DE. 2016. Bacterial spheroplasts as a model for visualizing membrane translocation of antimicrobial peptides. Antimicrob Agents Chemother 60:6350–6352. doi:10.1128/AAC.01008-16.