Date

2017

Department or Program

Biological Sciences

Primary Wellesley Thesis Advisor

T. Kaye Peterman

Additional Advisor(s)

Andrea Sequeira

Additional Advisor

Drew Webb

Additional Advisor

Andrew Schultz

Abstract

Unidirectional flow of the phytohormone auxin through files of cells occurs due to polar localization of PIN1 and is responsible for vascular development in Arabidopsis thaliana. The endosomal recycling pathway that establishes and maintains proper PIN1 localization is coordinated by phosphoinositide species within the cell’s membranes. In order to determine if the Sec14-like proteins PATELLIN1/2 and the related PATLELLIN6 are involved in regulating these phosphoinositide pools during vascular development, PATELLIN1/2 and other known vascular genes in this pathway or PATELLIN6 were knocked out and analyzed for evidence of genetic interactions. Analysis of the cotyledon venation of these higher order mutants revealed PATLELLIN1/2 interact with CVL1 and CVP2, but only weakly with VAB. Additionally, evidence showed PATELLIN6 plays a role in vascular development, but is not redundant with PATELLIN1/2. Therefore, this study provides evidence that PATELLIN1/2 play a role in regulating the PtdIns(4,5)P2 pools that are necessary for PIN1 endocytosis, while PATELLIN6 plays a unique role during vascular development.

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